Senior Technician - Harschnitz Group - Funded By Fis Milano

Senior Technician - Harschnitz Group - Funded By Fis
Milano
Lombardia, Milano

APPLICATION CLOSING DATE: January 29th, 2025 Human Technopole (HT) is an interdisciplinary research institute in Milan (Italy) created and supported by the Italian government to develop innovative strategies promoting human health.

HT adopts a multidisciplinary and integrated approach combining genomics, computational and structural biology, neuroscience, and data and decision sciences.

About the lab: The Harschnitz Group studies the molecular and cellular mechanisms that underlie susceptibility to viral and autoimmune disease of the human nervous system.

Its interests range from developmental neurobiology, human stem cell biology, and basic virology.

We develop and use cutting-edge human pluripotent stem cell (hPSC) technology to establish human in vitro disease models to study neuroinflammation in human, disease-relevant cells.

The group takes a multidisciplinary approach, combining our state-of-the-art human stem cell models with high-content chemical or genetic screens, single-cell analyses, and advanced high-resolution imaging technology to investigate host-virus interactions in the human brain.

The Harschnitz lab at Human Technopole is searching for a senior research technician to contribute to the new FIS Starting Grant: Grant n. FIS-2023-03708 CUP B53C24009410001 Project "Dissecting HSV-1 Latency and Reactivation using Trigeminal Organoids - 3D HSV" in the framework of the FONDO ITALIANO PER LA SCIENZA 2022-2023.

The successful candidate will be part of an interdisciplinary team that will investigate the host-virus interplay in the context of viral latency.

Specifically, they will report directly to Oliver Harschnitz while working in close collaboration with members of his team and the entire HT campus.

We are looking for highly motivated, independent, and creative stem cell biologists and virologists who have solid experience in human stem cell culture, CRISPR/Cas9 gene-editing, virology, and molecular biology.

Key responsibilities: Designing and performing experiments in the fields of stem cell biology and virology. Precise genome engineering of hPSCs using CRISPR/Cas technology. Establishment and optimization of new tools and protocols. Maintaining hPSC lines and hPSC-derived cell cultures. Generating reliable data for publication in the projects to which they are assigned and record all data and results in specified forms (paper and electronic). Providing technical support and supervision to other laboratory members, including introduction and teaching of new laboratory techniques and support of lab equipment. Participating in key aspects of general lab management and organization (e. g. , ordering/purchasing, managing inventories and stocks of samples, reagents, equipment, and materials). Mandatory requirements: PhD in a relevant subject area (stem cell biology, virology, neuroscience, or similar). Experience as Laboratory Technician. Experience in hPSC culture and differentiation. Strong molecular biology skills (e. g.

cloning, Western blot, RTq-PCR). Solid expertise in genome editing. Fluency in English - HT is an international research institute. Preferred requirements: Experience in immunocytochemistry and microscopy. Experience in virology and virus production/handling/infection. Organizational and social skills: Ability and interest to learn or develop new methodologies. Excellent communication skills. Proven independent working style, problem solving, and strong passion for science. Willingness and ability to work in an open, transparent, and highly collaborative team. Ability to work in an inclusive environment with sensitivity and respect for diversity. Any questions concerning the role and/or queries regarding the relevant terms and conditions should be addressed to ****** (this email address should not be used to send applications).

Application Instructions: Please apply online by sending:

a CV in English. a detailed motivation letter (up to 3 pages) in English clearly stating your motivation and fit. names and contact information of 2 referees. WHY HUMAN TECHNOPOLE: HT seeks scientific excellence.

We recruit the best scientific talents through international, open calls.

Our working environment is international, friendly, and inclusive.

Our scientists work together across disciplines on research topics of biomedical relevance, leveraging synergies between their diverse skill sets and methodological approaches.

We believe that highly diverse teams yield the best and most innovative results.

We engage in outward-facing scientific activities aimed at benefiting the national and international research community.

Training is also at the heart of our activity, with initiatives and opportunities for our staff, including scientific courses, conferences, and workshops.

MAIN BENEFITS: Welfare plans. Canteen service. Work-life balance provisions. Italian language training for foreigners. Parental leave up to 1 year and other support for new parents. Counselling. Flexible working hours. Support for relocation. Researchers coming to Italy for the first time, or returning after residing abroad, benefit from very attractive income tax benefits. Special consideration will be given to candidates who are part of the protected categories list, according to L. 68/99. Number of positions offered: 1 Starting date: MARCH 2025 Contract offered: CCNL Chimico Farmaceutico, Fixed-term 3 years.

Employee level.

Salary: € up to 41k The position is based in Milan.

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