Technician/Sr. Technician (Carninci Group) – Genomics Research Centre Milano

Technician/Sr. Technician (Carninci Group) – Genomics Research Centre
Milano
Lombardia, Milano

APPLICATION CLOSING DATE: March 14th, 2025 Human Technopole (HT) is an interdisciplinary life science research institute, created and supported by the Italian government, with the aim of developing innovative strategies to improve human health through a multidisciplinary and integrated approach, combining genomics, computational and structural biology, neuroscience, as well as population and health data science. The Carninci Research Group is seeking a Technician / Sr. Technician to join the team within the Research Centre for Genomics – Functional Genomics Programme , and reporting to Dr. Piero Carninci. About the lab The Carninci group strives to develop and broadly use technologies to comprehensively study the non-protein-coding part of the genome, its function and interactome. An overview of the laboratory mission is available at: Carninci Group - Human Technopole. Key tasks and responsibilities Conducting experiments in the area of functional genomics, combining cutting-edge methods from molecular biology, cell biology and genomics technologies, biochemistry, cell culture and, with a strong emphasis on development, optimization and application of functional genomics protocols and assaying long non-coding RNA functions; Generating, importing and optimizations of new tools and protocols; Generating reliable data for publication in the projects to which s/he is assigned and record all data and results in specified forms (paper and electronic); Providing technical support and supervision to other laboratory members, including introduction and teaching of new laboratory techniques and support on the use of lab equipment to lab members, as well as members from other laboratories; Participating in key aspects of general lab management and organization (e. g. , managing inventories and stocks of samples, reagents, equipment and materials). Essential requirements Considerable experience in laboratories involved in the area of main responsibilities, with particular emphasis on genomics. For a Senior Technician, at least 5 years of proven experience as Laboratory Technician or equivalent positions, including postdoctoral experience in relevant areas. Master in Biological Sciences or related field. Track record in the following areas: Functional Genomics libraries preparation and optimization for next generation sequencing; Molecular Biology (DNA and RNA manipulation, cloning, sequencing, RT-PCR and library preparation for next-generation sequencing applications), Protein Biochemistry (immunoblotting and immunoprecipitation techniques, preferably also recombinant protein expression, purification and in vitro reactions), Cell culture, transfection and knockdown experiments (siRNA, shRNA, antisense and similar technologies) to probe gene functions. Broad technical understanding of the area of genomics, next generation sequencing, cell culture, knock-down methodologies and other areas of main responsibilities. Fluency in English – HT is an international research institute. Preferential requirements Broad understanding of RNA biology, in particular of lncRNAs and other non-coding RNAs. Broad understanding of biochemistry and enzymology. Understanding of genome regulation in 3 dimensions. Previous experience in preparation of CAGE, full-length cDNA libraries, HiC or related technologies libraries. Experience in managing large projects involving preparation and tracking of large datasets. Experience in writing scientific literature in English, such as technical protocols and scientific publications. Required personal skills Ability to manage competing priorities in a fast-paced environment and to work independently. Strong interpersonal skills demonstrated by the ability to lead and gain the assistance and cooperation of others in a team endeavour. Ability to build trust through operating with transparency and creating an open and positive environment. Ability to work in a multi-cultural, multi-ethnic environment with sensitivity and respect for diversity. Application Instructions a CV a motivation letter in English the name and contact details of at least 2 potential referees. For specific enquires concerning the role only, please contact Piero Carninci, Head of the Research Centre for Genomics: pierohumantechnopole1. recruitee.com (this email address should not be used to send applications). Why Human Technopole HT seeks scientific excellence, we recruit the best scientific talents through international, open calls. Our working environment is international, friendly and inclusive. Our scientists work together across disciplines on research topics of biomedical relevance, leveraging synergies between their diverse skillsets and methodological approaches. We believe that highly diverse teams yield the best and most innovative results. We engage in outward-facing scientific activities aimed at benefiting the national and international research community. Training is also at the heart of our activity, with initiatives and opportunities for our staff, including scientific courses, conferences and workshops. Main benefits Welfare plans; Canteen service; Work-life balance provisions; Italian language training for foreigners; Parental leave up to 1 year and other support for new parents; Counseling; Flexible working hours; Remote working policy; Support for relocation; Special consideration will be given to candidates who are part of the protected categories list, according to L. 68/99. Number of positions offered: 1 Contract offered: CCNL Chimico Farmaceutico, 3 years employee level, with possibility of renewal. Salary range: up to 41 k depending on the seniority of the candidate. The position is based in Milan. The Foundation reserves the right, at its sole discretion, to extend, suspend, modify, revoke, or cancel this job posting without giving rise to any rights or claims whatsoever in favor of the candidates; the Foundation reserves, however, the right not to proceed with the awarding of the above-described assignment due to the effect of supervening regulatory provisions and/or obstructive circumstances. J-18808-Ljbffr