Technologist Position - 3D Chromatin And Non-Coding Rnas - (25000013) GENOVA

Technologist Position - 3D Chromatin And Non-Coding Rnas - (25000013)
Genova
Liguria, GENOVA

3D Chromatin Conformation and RNA Genomics

Organisation/Company: ISTITUTO ITALIANO DI TECNOLOGIA

Department: 3D Chromatin Conformation and RNA Genomics

Research Field: Biological sciences » Biology

Researcher Profile: Recognised Researcher (R2)

Positions: Postdoc Positions

Country: Italy

Application Deadline: 30 Mar 2025 - 10:02 (Europe/Rome)

Type of Contract: Temporary

Job Status: Full-time

Hours Per Week: 40

Is the job funded through the EU Research Framework Programme?

Not funded by a EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description Project: "Exploring the role of non-coding RNAs in 3D chromatin Hubs" – Acronym: 3D-Hubs – Grant Agreement number: FIS-2023-02410 – CUP: J53C24004180001 Contract: Fixed-term contract, full time Duration: up to 3 years THE ROLE You will be working in a multicultural and multi-disciplinary group, where junior and senior scientists collaborate, each with their expertise, to carry out a scientific activity with a shared research goal.

The "3D Chromatin Conformation and RNA Genomics " Research Unit is coordinated by Dr. Dafne Campigli Di Giammartino and aims at understanding how non-coding elements (such as enhancers and non-coding RNAs) and their epigenetic/epitranscriptomic modifications regulate 3D genome architecture and gene expression.

To address this question, we use cutting-edge chromatin conformation assays (e. g.

Hi-C, Hi-ChIP etc) in conjunction with other -omics techniques (e. g.

ChIP-seq, RNA-seq etc. )

and in combination with CRISPR-based genetic and epigenetic engineering tools in mouse stem cells as well as in human tumor stem cells.

Your main responsibilities will be: Using advanced molecular biology techniques for the manipulation of RNA, DNA and proteins in the area of 3D chromatin conformation, stem cells and non-coding RNAs Collaborating with other lab members to implement new technologies based on CRISPR/Cas platforms and biochemical assays for the characterization of the 3D chromatin architecture Supervising and mentoring junior members of the team Recording experimental data performed in the laboratory in both paper and electronic format (e. g.

benchling) Writing research and reviewing articles as well as assisting in generating preliminary data and figures for grant proposals ESSENTIAL REQUIREMENTS PhD in Cellular/Molecular Biology, Biochemistry or similar disciplines Extensive experience in a molecular biology, biochemistry, genetics or cell biology laboratory Previous experience in producing NGS data such as ChIP-seq, RNA-seq, ATAC-seq etc Experience with cell culture maintenance and complementary techniques (e. g.

FACS) Familiarity with CRISPR technologies Good publication record Strong command of spoken and written English Ability to work independently and collaboratively in a highly interdisciplinary environment ADDITIONAL SKILLS Experience in 3D chromatin conformation assays (e. g.

Hi-C, Hi-ChIP etc) Previous research experience in the RNA field Previous research experience in the stem cells field Ability to perform genomic and epigenomic editing in stem cells using CRISPR-(d)Cas technologies COMPENSATION PACKAGE A yearly gross salary ranging between 40K and 50K, depending on the candidate qualifications, which may include a bonus option depending on your role and contract Flexible working time Wide range of staff discounts Candidates from abroad or Italian citizens who have carried scientific research activity permanently abroad and meet specific requirements, may be entitled to a deduction from taxable income of up to 90% from 6 to 13 years.

Please submit your application using the online form and including: Your CV Contact info of 2-3 referees A 1 page Cover letter specifying your skills and motivations #J-18808-Ljbffr